Hepatic progenitor cells and uses thereof US 8415149 B2
Publication number US8415149 B2
Publication type Grant
Application number US 12/774,776
Publication date Apr 9, 2013
Filing date May 6, 2010
Priority date May 6, 2010
Also published as CN102234626A, US20110274664
Inventors Horng-Jyh Harn, Shinn-Zong Lin
Original Assignee Gwo Xi Stem Cell Applied Technology Co., Ltd.
What is claimed is:
1. A composition comprising a preparation containing cultured pluripotent animal cells that are (i) positive for CD29, CD44, CD49b, CD49d, CD73, CD90, CD105, and CD 13; and (ii) negative for CD14, CD34, HLA-DR, and CD45; and a liver tissue.
2. The composition of claim 1, wherein the liver tissue is an isolated liver tissue.
3. The composition of claim 1, wherein the liver tissue is an injured liver tissue has been subjected to a hepatotoxic agent.
4. The composition of claim 1, wherein the pluripotent animal cells are obtained from the Wharton's jelly of the umbilical cord of a first subject.
5. The composition of claim 4, wherein the first subject is a human.
6. The composition of claim 4, wherein the liver tissue is obtained from a second subject.
7. A method for obtaining hepatic progenitor cells, comprising culturing the composition of claim 1 in a medium for a period of time; and collecting progenies of the cultured pluripotent animal cells to obtain hepatic progenitor cells, wherein the pluripotent animal cells are obtained from the Wharton's jelly of the umbilical cord of a first subject.
8. The method of claim 7, wherein the hepatic progenitor cells express one or more of genes selected from the group consisting of CK18, albumin, tryptophan 2,3-dioxygenase (TO), afetoprotein (AFP), CYP7A1, nanog, oct4, ckit, HGF, and MMP.
9. The method of claim 8, wherein the pluripotent animal cells are positive for HGF or MMP.
10. The method of claim 7, wherein the first subject is a human.
11. The method of claim 7, wherein the liver tissue is an injured liver tissue.
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