Human Induced Pluripotent Stem Cell (HiPSC) Workshop by ALEXEY BERSENEV on JUNE 7, 2012 ·This is a guest post by Stacey Johnson Toronto's Centre for Commercialization of Regenerative Medicine (CCRM) is offering a practical course in how to produce and maintain iPSCs. Participants will also learn about standardized validation and characterization methods to ensure the quality of the stem cell lines.
Workshop Key Learning Objectives: Theory of iPSC production and underlying technical methodologies applied to achieve reprogramming. Hands-on basic cell culture for human ESCs, iPSCs and primary fibroblast lines, including media preparation, colony picking and expansion, cryopreservation and revival. Hands-on induction of fibroblasts and blood cells using various transgene delivery methods. Hands-on identification of emergent iPSC colonies and their isolation. Characterization of established iPSCs via immunostaining and quantitative PCR, as well as
high-throughput methods using flow cytometry. Differentiation of iPSCs along desired embryonic lineages. Please register by July 13, 2012 as space is limited. The workshop takes place August 13-15 in Toronto. More details can be found here.
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